Both Bioremediation and Biodegradation are microorganism governed processes. They deal with the hazardous chemicals prevailing in our environment. But both of them differ widely based on their functioning and biotreatment potential. Biodegradation is a self occurring process. This process biotransforms the toxins into their simpler non-toxic form by using microorganisms. It is a slow process that doesn't require any human intervention. Whereas Bioremediation is a human-driven process to … [Read more...]
Difference Between PCR, RT-PCR and qPCR
Beginning genetic material i.e., the template is different in all three types of PCR- regular, RT PCR and qPCR. The basic PCR utilizes normal double-stranded DNA as a template. Whereas RT PCR permits only works with RNA as a template. qPCR is the most advanced version that is capable of working with both DNA and RNA as templates. PCR and RT PCR only determine the presence or absence of a specific genetic fragment in a sample. However, qPCR has the competence to state the absence or … [Read more...]
Difference Between Southern, Northern and Western Blotting Techniques
Southern blotting is the first detection technique which is used to identify the specific DNA sequences in the provided tissue or blood sample and is named after the scientist who developed it, 'Ed Southern in the year 1975. Northern blotting is the technique used to know the gene expression of the provided sample by identifying the specific RNA or mRNA molecules; it was developed by David Kemp, James Alwine and George Stark in 1977 at Stanford University. Western blotting is a sensitive … [Read more...]
Difference Between Bioreactor and Fermenter
A system in which the organisms are cultivated and provoked to produce the desired products is called a bioreactor. In contrast, the fermenter is the system used for the fermentation process to produce the desired outcomes. Mainly in the bioreactor, plant and animal cells are used, while fermentation is limited to microbial use only. These are types of closed- system/ vessels that are used for the particular type of biochemical reaction, where, in the bioreactor, various kinds of biochemical … [Read more...]