The key difference among PCR, RT PCR and qPCR is the beginning genetic material, i.e. the template used for the reaction. DNA as a template is utilised in fundamental PCR, whereas RT PCR permits only RNA to be used as a template. On the other hand, qPCR is capable of using both DNA and RNA as templates. PCR and RT PCR techniques can only determine the presence or absence of a specific genetic fragment in a sample. However, qPCR has the competence to not only indicate the absence or presence … [Read more...]
Difference Between Southern, Northern and Western Blotting Techniques
Southern blotting is the first detection technique which is used to identify the specific DNA sequences in the provided tissue or blood sample and is named after the scientist who developed it, 'Ed Southern in the year 1975. Northern blotting is the technique used to know the gene expression of the provided sample by identifying the specific RNA or mRNA molecules; it was developed by David Kemp, James Alwine and George Stark in 1977 at Stanford University. Western blotting is a sensitive … [Read more...]
Difference Between Bioreactor and Fermenter
A system in which the organisms are cultivated and provoked to produce the desired products is called a bioreactor. In contrast, the fermenter is the system used for the fermentation process to produce the desired outcomes. Mainly in the bioreactor, plant and animal cells are used, while fermentation is limited to microbial use only. These are types of closed- system/ vessels that are used for the particular type of biochemical reaction, where, in the bioreactor, various kinds of biochemical … [Read more...]